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. 2017 Oct 18;7:13462. doi: 10.1038/s41598-017-13857-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The location of the pemIK-Sa6 operon in recently described composite staphylococcal cassette chromosome SCCmec _AI16-SCCczr _AI16-CI of S. pseudintermedius AI16 and a contig of unknown context that was derived from S. aureus UCIM6147. The genes within the composite SSC are illustrated after Chanchaithong et al., excluding the pemIK locus and two adjacent open reading frames, which were considered hypothetical proteins²⁹. White arrows indicate sequences that encode hypothetical proteins, and coloured arrows indicate the following genes: orfX, grey; mec operon, sky blue; cadium resistance, pale green; cyclopentanol dehydrogenase, brick red; ccrB3 and ccrA1 recombinase genes, green and blue; transposases of Tn554 family, light grey; type I restriction-modification system, orange; czr operon, brown; ccrA1, ccrB3, and ccrB6 recombinase as blue, green, and cyan, respectively. In the sequence of S. aureus UCIM6147: crossover junction endodeoxyribonuclease, rusA, magenta; putative ATPase, yellow; XRE family transcriptional regulator and a gene located downstream of putative non-specific DNA/RNA NUC endonuclease, dark blue. In the fragment containing the pemIK locus: tccA, teicoplanin resistance-related protein, dark purple; pemIK operon, red; and putative divalent ions transporter, dark green.