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. 2017 Oct 18;7:13494. doi: 10.1038/s41598-017-13994-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PEG10 is overexpressed in chondrosarcoma cell lines SW1353 and Hs 819.T. (a) Expression of PEG10 in the indicated cell lines was examined by RT-qPCR (n = 3). (b) A mixture of four independent siRNAs against PEG10 was transfected into the indicated cells, followed by immunoblotting for PEG10. A specific band for PEG10-RF1, which was diminished by knockdown, is indicated. Tubulin served as a loading control. The blot for tubulin was cropped and the full-length blot is presented in Supplementary Figure S5.