Table 1.
Results of microarray analysis: genes upregulated in chondrosarcoma cells and downregulated by TGF-β1 treatment.
| Gene symbol | Gene description | probe signal intensity | ||||
|---|---|---|---|---|---|---|
| C28/I2 + mock | SW1353 + mock | SW1353 + TGF-β1 | Hs 819.T + mock | Hs 819.T + TGF-β1 | ||
| CEACAMP6 | carcinoembryonic antigen-related cell adhesion molecule pseudogene 6 | 11.644251 | 849.7766 | 293.17218 | 805.261 | 188.41696 |
| CHI3L1 | chitinase 3-like 1 (cartilage glycoprotein-39) | 30.32458 | 891.2907 | 193.51378 | 733.03796 | 274.57275 |
| DUSP10 | dual specificity phosphatase 10 | 264.37048 | 1730.9897 | 749.4703 | 1657.7657 | 691.1046 |
| F2RL2 | coagulation factor II (thrombin) receptor-like 2 | 51.60757 | 1596.104 | 743.29755 | 2073.8364 | 792.52045 |
| GPNMB | glycoprotein (transmembrane) nmb | 69.241974 | 456.36475 | 134.65535 | 437.88937 | 143.89958 |
| IL36B | interleukin 36, beta | 14.552366 | 708.716 | 261.0168 | 402.83282 | 134.94543 |
| MAD1L1 | MAD1 mitotic arrest deficient-like 1 (yeast) | 170.33104 | 1065.3365 | 415.22104 | 1033.9989 | 438.05844 |
| MIR221 | microRNA 221 | 91.36347 | 869.62396 | 263.8181 | 1107.2083 | 283.76727 |
| MME | membrane metallo-endopeptidase | 30.921976 | 613.5276 | 265.4864 | 757.2124 | 338.64307 |
| MMP3 | matrix metallopeptidase 3 (stromelysin 1, progelatinase) | 20.330805 | 878.2055 | 197.87737 | 1292.2312 | 266.2604 |
| MOSPD1 | motile sperm domain containing 1 | 196.03433 | 1332.8429 | 626.03235 | 1398.9138 | 680.8544 |
| PDE5A | phosphodiesterase 5 A, cGMP-specific | 14.682203 | 415.84045 | 98.5719 | 706.4834 | 166.55884 |
| PEG10 | paternally expressed gene 10 | 92.65147 | 460.203 | 206.1215 | 459.444 | 197.3221 |
| PHLDA1 | pleckstrin homology-like domain, family A, member 1 | 103.9472 | 489.51953 | 215.93834 | 433.7974 | 207.7058 |
| PSG4 | pregnancy specific beta-1-glycoprotein 4 | 28.558039 | 1003.54065 | 367.46582 | 1020.25616 | 259.7936 |
| RAB27B | ras-related protein Rab-27B | 57.8036 | 1387.0364 | 239.84311 | 1366.6107 | 252.79196 |
| VAT1L | vesicle amine transport protein 1 homolog (T. californica)-like | 165.3608 | 801.96643 | 307.95685 | 824.4419 | 367.76886 |
C28/I2 cells were mock treated, while SW1353 and Hs 819.T cells were treated with or without TGF-β1 (1 ng/ml) for 48 h, followed by mRNA purification and microarray analysis. Genes were identified based on a 4-fold higher probe signal in both SW1353 and Hs 819.T cells compared with C28/I2 cells with notable intensity of >400, which decreased to less than 0.5-fold upon TGF-β1 induction.