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. 2017 Oct 18;7:13456. doi: 10.1038/s41598-017-13917-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of selected proteins in LSECs from NaAsO₂-induced LSEC dedifferentiation and CCl₄-induced liver injury mice model. (A) Differential expressions of 2 up-regulated proteins (CLTC, Dab2) and 3 down-regulated proteins (Galectin-3, SAMHD1, Rab-10) were validated by Western blot. The relative ratios of proteins by iTRAQ analysis were shown right. All western blot experiments were repeated at least three times. Full-length blots are included in Supplemental Information. (B) Representative SEM images of liver sinusoids (n = 5) and quantitative porosity of sinusoidal fenestrae at 0 and 6^th week with CCl₄ administration. Bar = 1μm. (C) Masson’s trichrome staining and αSMA expression in liver at 6^th week with CCl₄ administration (n = 5) were analyzed by immunohistochemistry analysis, Bar = 100μm. (D) Western blot analysis of primary LSECs isolated from 0 and 6^th week for the levels of CLTC, Dab2, Galectin-3, SAMHD1 and Rab-10 proteins, and GAPDH was used as loading control. All western blot experiments were repeated at least three times. Full-length blots are included in Supplemental Information. (E) CLTC expression in liver at normal control mice and mice at 6^th week with CCl₄ administration (n = 5) were analyzed by immunohistochemistry analysis, Bar = 100μm.