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. 2017 Oct 18;7:13424. doi: 10.1038/s41598-017-13949-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of P-BBG diet on levels of myocardial p53 and Parkin. (A) P-BBG diet (n = 7) increased the myocardial protein levels of p53 compared to Control (n = 7) diet. Levels of p53 are expressed as arbitrary units of p53 (53 kDa, MW)/GAPDH (37 kDa, MW) ratio. Representative images of cropped densitometric bands of p53 and GAPDH are shown. The full-length blots/gels are presented in Supplementary Figure 1F. (B) P-BBG diet (n = 7) increased the myocardial protein levels of Parkin compared to Control diet (n = 7). Levels of Parkin are expressed as arbitrary units of Parkin (52 kDa, MW)/GAPDH (37 kDa, MW) ratio. Representative images of cropped densitometric bands of Parkin and GAPDH are shown. The full-length blots/gels are presented in Supplementary Figure 1G. (C) Representative images of Parkin localization in coronary endothelium of Control left ventricles. (D) Representative images of Parkin localization in coronary endothelium of P-BBG left ventricles. P-BBG: low fat diet supplemented with barley beta-D-glucan enriched pasta (3 g/100 g of dry weight); GAPDH: glyceraldehyde 3-phosphate dehydrogenase. All measurements are mean ± SD. *p < 0.05 vs. Control.