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. 2017 Oct 18;7:13433. doi: 10.1038/s41598-017-13538-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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3D tomographic reconstruction of NK cell interaction with starving HEK293T cell. (a,b) Two slices from the reconstructed 3D volume of two NK cells interacting with a starved HEK293T cell. Vacuoles (v), nuclear envelope (ne), and carbon-dense vesicles (cd) are marked. The full volume and a segmentation are provided in Supplementary Material. (c) One of the 2D 10-second projections used for the tomography, for comparison. Scale bar 10 µm.