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. 2017 Oct 18;7:13442. doi: 10.1038/s41598-017-13880-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A prolonged LMB treatment (16 h) of macrophages induces a state of severe oxidative stress. (a) Western blot analysis in the expression of proteins c-Jun, c-Myc and β-adaptin present in the nuclear fraction after a prolonged LMB treatment in the presence of natLDL and oxLDL. (b) Under the same conditions, cell viability obtained by MTT assays. (c) LMB effect upon internalization of dil-fluorescently labeled natLDL and oxLDL. (d) ROS characterization under the same conditions. Mean values are presented (n = 6, X ± SD) *p < 0.05, **p < 0.01.