Figure 4.

Subcellular localization of Rs-2b and its mutants detected by BiFC in Nicotiana benthamiana plants. N. benthamiana plants were co-agroinfiltrated with transiently expressed 2b proteins fused to either the N or C terminus of yellow fluorescent protein (YFP). Rs-CMV was clearly detectable in cytoplasm as well as in nuclei (A,B). No fluorescence was observed by confocal microscopic imaging in cells within leaf patches co-infiltrated with A. tumefaciens cells expressing 2b/40-42/AAA. In the case of mutant 2b/40-42/DPD fluorescence was detectable only in cytoplasm, not in the nucleus, nor in the nucleolus (A,B). The bar represents 50 µm (A) or 25 µm.