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. 2017 Oct 18;7:13482. doi: 10.1038/s41598-017-14027-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Treatment with nt-p65-TMD reduces inflammatory mediators in BV2 microglia and RAW264.7 macrophages and elevates anti-inflammatory mediators in the RAW264.7 macrophages. (a) The culture supernatants from the BV2 cell line were collected 24 h after LPS stimulation and the IL-1β (7 repeats), IL-6 (7 repeats), and TNF-α (7 repeats) levels were evaluated by ELISA. (b) The transcripts of anti-inflammatory mediator genes such as arginase-1 (7 repeats) and IL-10 (7 repeats) and (c) pro-inflammatory mediator genes such as IL-1β (4–5 repeats), IL-6 (6 repeats), TNF-α (6–7 repeats), CXCL10 (7 repeats), and iNOS (7 repeats), of BV2 microglia were measured by real-time PCR. (d) The culture supernatants from the RAW264.7 cell line were collected 24 h after LPS stimulation, and the IL-1β (7 repeats), IL-6 (5 repeats), and TNF-α (5 repeats) levels were assessed by ELISA. (e) The transcripts of anti-inflammatory mediator genes, such as arginase-1 (5–6 repeats) and IL-10 (5 repeats), and (f) pro-inflammatory mediator genes, such as IL-1β (5–7 repeats), IL-6 (5 repeats), TNF-α (7 repeats), CXCL10 (7 repeats), and iNOS (6 repeats), of RAW 264.7 macrophages were calculated by real-time PCR. Values are means ± SEM. P values were calculated with multiple comparisons by Bonferroni tests. *p < 0.05, **p < 0.01, ***p < 0.001 vs. LPS. Statistical parameter (Supplementary Table 7).