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. 2017 Oct 18;7:13440. doi: 10.1038/s41598-017-13397-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qPCR validation of differentially expressed genes in adult T. urticae females after PBO, DEF or CsA treatment. Eight up- and five downregulated genes as assessed by differential gene expression (DESeq2) analysis of RNA-seq data were selected for qPCR analysis. Error bars represent the standard error of the calculated mean. Except for tetur40g00030 (CsA treatment), each gene was significantly differentially expressed (based on an unpaired t-test) compared to the reference condition (FORM). The ‘‘tetur’’ prefix was removed from T. urticae gene IDs for figure clarity.