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. 2017 Oct 18;7:13504. doi: 10.1038/s41598-017-14077-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Primary structure of WSD isoforms in E. gracilis. (A) Molecular organization of six WSD isoforms. Designations of putative domains were based on Pfam domain database: WES, wax ester synthase-like acyl-CoA acyltransferase domain; DUF1298, domain of unknown function. (B) Comparison of putative active site residues of WSDs from Euglena (EgWSD1-6, LC069357-LC069364), Arabidopsis (AtWSD1, At1G72110) and Acinetobacter calcoaceticus ADP1 (AcWS/DGAT, AF529086) using the ClustalW program. Amino acids matching the putative active site motif HHxxxDG are shaded in black. (C) Phylogenetic analysis of the six WSDs from Euglena and proteins related to WSD from other organisms. Phylogenetic trees were constructed with full-length WSD amino acid sequences using the neighbor-joining (NJ) method using the MEGA 6.0 software (http://www.megasoftware.net/). Sequence alignments were assembled by the ClustalW algorithem version 2.1. Abbreviations and UniProt accession numbers except for EgWSDs are as follows: At, Arabidopsis thaliana (AtWSD1-11, Q93ZR6, Q9C7H4, F4IU14, Q9M3B3, Q9M3B2, Q9M3B1, Q94CK0, Q9FFE8, Q9FK89, Q9FK04, Q5KS41); Bo, Brassica oleracea var. oleracea (A0A0D3APY8); Br, Brassica rapa (A0A078CR28); Cr, Capsella rubella (R0IEG2); Ab, Acinetobacter baumannii (A0A077GKS2); As, Amycolicicoccus subflavus DQS3-9A1 (F6EKR7); Mh, Marinobacter hydrocarbonoclasticus (A3RE50); A.baylyi sp.ADP1 aft, Acinetobacter baylyi sp. ADP1(Q8GGG1); Mt, Mycobacterium tuberculosis (P9WKC7); Ra, Rhodococcus aetherivorans (A0A1Q8I8D0); Mf, Myxococcus fulvus 124B02 (A0A0F7DYG7); Ma, Mucor ambiguus (A0A0C9N7W4); Pg, Photobacterium ganghwense (A0A0J1HAU3).