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. 2017 Oct 18;7:13504. doi: 10.1038/s41598-017-14077-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative expression analysis of WS and WSD genes in response to aerobic and anaerobic conditions. Total RNA was extracted from 7-d-old Euglena SM-ZK cells grown heterotrophically under normal growth conditions (white bar) and the cells anaerobically treated for 24 h (blue bar). Quantitative PCR analysis was performed to determine the expression levels of the indicated genes. Relative expression levels were normalized to malate synthase mRNA. Values are the mean ± SD of three independent measurements.