Figure 6.

GC-TOF/MS-based lipid profiling between mock control and WSD2/5-KD. Aerobically grown Euglena cells were placed under anaerobic conditions for 24 h. The Euglena cells were collected at 0 h and 24 h. Total lipid was extracted from the Euglena cells and dried using a centrifugal evaporator. The non-methylesterified lipid fractions prepared from the mock control and WSD2/5-KD cells were analyzed by GC-TOF/MS. Wax ester contents were determined by a GC-TOF/MS-based profiling method as described by Furuhashi et al.²⁴. (A) Representative GC-TOF/MS total ion chromatograms (TIC) of mock-control and WSD2/5-KD Euglena cells treated with anaerobic conditions for 24 h. (B) Comparison of relative amount of wax ester molecules between mock control and WSD2/5-KD. The data was processed based on the region enclosed by the dashed line. Asterisks indicate statistically significant differences between mock-control and WSD2/5-KD Euglena cells (means ± SD, n = 3; biological replicate, Student t-test, *p < 0.05).