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. 2017 Oct 18;7:13473. doi: 10.1038/s41598-017-14063-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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No significant autophagic accumulation in Pom iPSC^MyoD-derived myocytes. (a) Representative microscopic images of immunofluorescence for LC3 (red; autophagic marker) and MHC (green) in iPSC^MyoD-derived myocytes. Pom iPSC^MyoD-derived myocytes were cultured without or with 1 µM rhGAA for 3 days. Nuclei were stained with DAPI. Scale bar = 20 µm. (b) MFI of LC3 (integrated LC3 fluorescent signals adjusted for MHC-positive area) in iPSC^MyoD-derived myocytes (left graph) and the fold change of MFI with rhGAA rescue (right) (n = 3 microscopic fields). (c) Representative blots of Western blot analysis for LC3 in protein extracts from iPSC^MyoD-derived myocytes. Abbreviation: exp., exposure. (d) Quantitative densitometric analysis of western blots (n = 3 experiments). The left graph shows LC3-II/LC3-I ratio in iPSC^MyoD-derived myocytes. The right graph shows fold change of LC3-II/LC3-I ratio with rhGAA rescue.