Table 1.

Primers, probes, and amplicon sizes of the rRT-PCR assays.

Group	NA subtype	Forward Primers (5′-3′)	Reverse Primers (5′-3′)	Probes (5′-3′)	Amplicon size(bp)
1	N1	TGTGTGTGCAGRGAYAAYTGSC	GGACCRCAACTSCCTGTHCCRTCV	FAM-CCACGCCCCAATGATGGAACAGGCAGTTG- BHQ1	137
	N4	ATGGTGTTTGGATAGGGAGGACAAA	CACCATTTGAATCCTTGTCTGTCGA	HEX-AGCTTGGAATCCAGAAGCGGTTTTGAGATGGT- BHQ1	105
	N5	AGAACACAAGAGTCTTCGTGTGTTT	TGGGATAACAGGAACATTCTTCAAT	TexasRed-AGAGTGTTATTGGGTAATGACGGACGGTCCA- BHQ1	178
2	N3	AGCAGTTGCTTCGATGGAAAG	CATTGACATTCAGACTCTTGAGTTC	FAM-ATGACCGGGAACGACAATGATGCGAGTGG- BHQ1	155
	N2	ACGAGTTGGGTGTCCCGTTTCATTT	TTCTATCATCCCCAGTGACACAAAC	HEX-TGGGAACCAAACAAGTGTGCATAGCATGGTC- BHQ1	117
	N6	AAGGGTGCAGGATGTTTGCTCTAAG	CCTAGTATTATATGGACTGGGTGCT	TexasRed-AAGGCACAACACTCAGAGGGCGACATGCAAAT- BHQ1	140
3	N7	ACTCAGGAGTCAGAATGTGTATGCC	TCAATATGTTTGGCTGATCCCTTTA	FAM-TGGCACATGTGCAGTTGTAATGACTGACGG- BHQ1	155
	N9	GCCCTGATAAGCTGGCCACT	TGCATTGTTGTTTGGTCCTGATATA	HEX-ATCACCGCCCACAGTGTACAACAGCAGGGT- BHQ1	117
	N8	AAGAAGTGGATGACGATTGGTGTAA	AGTCCTTAATATATCTCCTGCCCAG	TexasRed-CAGGGCCAGATTCTAAAGCAGTAGCAGTAG- BHQ2	138

Codes for mixed bases position: R = A/G, Y = C/T, S = G/C, H = A/C/T, V = A/G/C.