Table 5.

Detection limits of the single and multiplex assays for 9 NA subtypes.

Group	NA subtype	Fluorescent dye	Limits of detection (EID50/PCR)
			Single assays	Multiplex assays
1	H5N1	FAM	1	10
	H1N4	HEX	10	10
	H5N5	Texas Red	1	10
2	H10N3	FAM	10	100
	H5N2	HEX	10	100
	H4N6	Texas Red	100	100
3	H10N7	FAM	1	10
	H7N9	HEX	10	100
	H3N8	Texas Red	10	10