Fig. 4.

Peroxiredoxin-2 mutants no longer benefit from developmental cultivation at 25 °C. A, Wildtype (dashed lines) and prdx-2 mutants (solid lines) were grown at 25 °C or 15 °C (as in Fig. 1) and shifted to the opposing temperatures as late L4 larvae. The animals that remained at 25 °C during development and adulthood (red line) had a shorter lifespan than animals kept constantly at 15 °C (blue line). In contrast to wildtype animals (dashed green line), prdx-2 mutants shifted from 25 °C to 15 °C at the end of L4 larval stage (solid green line) do not show an extended adult lifespan. A representative lifespan assay is shown. B, The average of the median lifespan of the prdx-2 mutants of all 4 experiments and the SEM are shown. The four subpopulations were compared with each other and means that are not significantly different, share the same letter (ANOVA Tukey multiple comparisons test, p<0.0001). Number of animals in each experiment for 15 °C, N2: 67, 16, 88, 78 and prdx-2: 58, 60, 83, 54; for 25 °C, N2: 69, 106, 86, 81 and prdx-2: 64, 60, 67, 83; for the 25 °C − 15 °C switch, N2: 72, 89, 81, 80 and prdx-2: 59, 64, 84, 64; and for the 15 °C − 25 °C switch, N2: 60, 56, 84, 81 and prdx-2: 62, 57, 83, 86. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)