Figure 3.

SAXS data demonstrate different levels of disorder in ColN constructs. (A) Shown here is an R_g-based, dimensionless Kratky plot where R_g is the radius of gyration Å, q is the momentum transfer or scattering vector Å⁻¹, I(q) is the scattered intensity at the given value of q, and I₀ is the scattering intensity at q = 0. The plot shows that the degree of disorder in colicin constructs increases with the length of the T-domain and that the K145A mutant has a higher level of disorder compared with WT. The data from a virtually globular colicin domain ColA-P are shown for comparison; ColN-RP, which lacks the disordered T-domain, is more asymmetric than the isolated P-domain, and this is reflected in the shape of the plot. (B) The distance distribution function P(r) displays elongated shapes in all constructs that contain the T- domain. The same color code/line type is used for each sample on both panels. To see this figure in color, go online.