Figure 3.

Membrane curvature was engineered by draping a supported lipid bilayer over NPs. (A) The 70 nm radius fluorescent NPs on glass were imaged with λ_ex = 488 nm. (B–E) The membrane was imaged with λ_ex = 561 nm and the differences between the polarizations provide internal controls. (B and C) Diffraction-limited p-polarized and s-polarized TIRFM image, respectively. (D and E) Reconstructed images of the membrane over the NPs presented as 2D histograms of the localizations in p-PLM and s-PLM, respectively. (F–J) Magnified views of the dashed regions from (A–E) show membrane curvature coincident with the NPs. (I) The detected membrane curvature over the 70 nm NPs is indicated by white arrows. (I and J) A multicolored fiduciary mark is indicated by red arrows. Scale bars represent (A–E) 5 μm and (F–J) 400 nm. The Supporting Material provides similar results for other size and color NPs (Figs. S4 and S5), LUVs (Fig. S6), GUVs, (Fig. S7), and other magnifications of these data (Fig. S13). To see this figure in color, go online.