(A–C) Radial probability distribution of localizations versus distance from the center of the curved membrane (r) over NPs of rNP = 24, 51, and 70 nm, respectively. Gray lines represent individual events and the colored lines represent the averages. Error bars represent the mean ± SE at a given r. (D–F) Histograms of the radius for each membrane curvature event (<r>) for rNP = 24, 51, and 70 nm. Black lines represent the Gaussian fits to guide the eye. The mean of the events radii was 32 ± 4, 50 ± 14, and 60 ± 13 nm for rNP = 24, 51, and 70 nm, respectively. To see this figure in color, go online.