Figure 7.

Single-particle tracking of DiI molecules reveals slowed diffusion at the site of nanoscale membrane curvature equally while imaged with p-polarized or s-polarized excitation. SLBs were draped over 70 nm radius NPs. Particle locations were projected on the xy-plane and the apparent fluorophore diffusion was affected by both the 3D membrane topology and the influences of membrane curvature on DiI mobility. The fit of the distribution of step lengths to Eq. 1 yielded the apparent diffusion coefficient and the 95% confidence range, as indicated by the error bars. Neither a locally Brownian diffusion nor a simulated barrier to free diffusion surrounding the bud reproduced the experimental results. However, on simulating a decreased local D for curved SLB slower than that of the planar SLB, the resulting simulated D_xy matched the experimentally observed D_xy. Simulations matched experimental data when the planar SLB had a D that was 25 ± 5× faster than the curved SLB. To see this figure in color, go online.