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. 2017 Aug 28;14(5):4448–4452. doi: 10.3892/etm.2017.5058

Figure 2.

Figure 2.

MiR-148a regulates HLA-G expression in the EC9706 cell line. (A) Position 376–382 of the HLA-G 3′UTR is a putative target site of miR-148a. (B) Relative mRNA level of HLA-G in cells transfected with miR-148a mimic or control vectors (NC or BC). The expression level of HLA-G mRNA was normalized to that of GAPDH. (C) Protein level of HLA-G detected by western blotting in cells transfected with miR-148a mimic or control vectors. GAPDH was used as an internal control. **P<0.01 vs. BC group. UTR, untranslated region; miR, microRNA; NC, negative control; BC, blank control; HLA-G, human leukocyte antigen-G.