Figure 3.

MiR-148a increases the rate of apoptosis in EC9706 cells. Cells were transfected with miR-148a mimic or control vectors (NC or BC). Cells (~5×10⁵ per treatment) were stained with Annexin V-fluorescein isothiocyanate and propidium iodide followed by flow cytometry analysis. **P<0.01 vs. BC group. miR, microRNA; NC, negative control; BC, blank control; Q1-4, quartiles 1–4. Percentages in quartiles 1–4 represent cells stained as annexin V⁻/PI⁺ (necrotic cells), annexin V⁺/PI⁺ (late apoptotic), annexin V⁻/PI⁻ (alive cells) and annexin V⁺/PI⁻ (early apoptotic), respectively.