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Schematic overview of the current study. (a) 10, 16, and 20 μm thick FF human uterus tissue were used in the study. (b) Proteins from the tissues were extracted using 3 different solutions varied by detergent (SDS) and organic solvent (ACN). (c) Protein digestion was performed over 18 h using a single portion of trypsin (Protocols 1, 2, and 4) or for an additional 4 h after the addition of second portion of trypsin (Protocols 3 and 5).
