Figure 3.
The super-resolution capabilities of PLM and dSTORM revealed membrane bud size (rbud) versus CTxB incubation time. As some buds grew to a larger diameter or into tubules (Figs. 4 and S3), new small buds (rbud < 50 nm) continued to form, and the distribution of rbud widened over time. The number and size of the buds increased as the CTxB incubation time increased. This is shown by (A) histograms and (B) whisker plots of the bud sizes observed in both the pPLM and CTxB channels. (C and D) Autocorrelation analysis of pPLM and CTxB localizations as a function of incubation time also shows the bud formation dynamics without the threshold-based identification of individual buds. (E) Cross-correlation analysis of pPLM and CTxB localizations shows the correlated localization of CTxB and membrane bending. To see this figure in color, go online.