Figure 6.

Single-particle tracking was performed on both (A) DiI and (B) CTxB as a function of position within a membrane bud. Before the CTxB is added (t = 0 min), no CTxB was located on the SLB, and no membrane buds were present. Random locations in PLM were chosen to confirm that our analysis routines demonstrated no significant variation in D_xy versus distance away from those locations. At all later times, a significant slowing of both the DiI and CTxB diffusion was observed within 50 nm of the bud center. The error bars represent at a 95% confidence interval of the fitting of Eq. 1 to the histograms of single step lengths. The step lengths were binned based on the distance from the bud center to the mean of the two linked localizations. To see this figure in color, go online.