Figure 7.

When membrane buds were formed by draping SLBs over nanoparticles of known size (70 nm radius), the sorting of CTxB versus membrane curvature could be determined. (A) Many combinations of the fitting parameters yielded quality fitting to the experimental data. (B) The membrane topography over the nanoparticle could be approximated to connect the concentration of CTxB per membrane area to the acquired z-projected data. The distribution of adequate model fits to the experimental data yielded a mean and SD of CTxB concentration on the membrane top and neck relative to the planar SLB; these were (12 ± 4) and (26 ± 11), respectively, with the median, quartiles, and range of CTxB concentrations shown in (C). To see this figure in color, go online.