Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
(a) Schematic view of the biosensor for NE activity. The biosensor is formed by the YFP-CFP FRET pair connected by a linker peptide carrying the NE-specific recognition sequence Phe-Ile-Arg-Trp. The GFP variants YFP and CFP are represented using the available three-dimensional structure of the wild-type GFP (PDB: 1EME). (b) Fluorescence emission spectra of the biosensor in the intact and cleaved form obtained by incubation with NE. To see this figure in color, go online.
