Table 1.

Dynamic MT Parameters for Growth and Catastrophe

		Growth			Shortening		Catastrophe
	N	Rate (nm/s)	Duration (s)	Length (μm)	Rate (nm/s)	Duration (s)	Frequency (min−1)
No motor	230	16.8 ± 0.3	165.2 ± 6.8	2.5 ± 0.1	352.7 ± 10.8	7.9 ± 0.3	0.24 ± 0.01
KIF3CC (50 nM)	252	24.5 ± 0.8	95.2 ± 6.6	1.5 ± 0.1	513.6 ± 20.7	2.9 ± 0.1	0.47 ± 0.04
No nucleotide	194	16.7 ± 0.4	174.1 ± 7.5	2.6 ± 0.1	474.0 ± 14.9	6.0 ± 0.2	0.27 ± 0.01
2 mM MgAMPPNP	229	19.4 ± 0.4	183.6 ± 7.4	3.3 ± 0.1	709.4 ± 20.0	4.9 ± 0.2	0.24 ± 0.01
KIF3AB (50 nM)	201	17.3 ± 0.4	153.7 ± 6.9	2.4 ± 0.1	460.6 ± 15.4	6.0 ± 0.3	0.24 ± 0.01
KIF3AC (50 nM)	177	18.1 ± 0.5	146.3 ± 6.7	2.3 ± 0.1	454.4 ± 14.2	5.8 ± 0.2	0.24 ± 0.01
KIF3CCΔL11 (50 nM)	186	19.5 ± 0.5	162.1 ± 6.9	2.8 ± 0.1	451.6 ± 14.8	7.6 ± 0.3	0.26 ± 0.01
KIF3CC
1 nM	189	20.4 ± 0.4	156.6 ± 7.0	2.8 ± 0.1	375.4 ± 14.8	8.9 ± 0.4	0.26 ± 0.01
1.5 nM	187	20.2 ± 0.5	150.8 ± 6.5	2.6 ± 0.1	403.1 ± 11.5	7.1 ± 0.2	0.27 ± 0.01
2 nM	330	18.8 ± 0.5	179.6 ± 6.0	2.8 ± 0.1	310.7 ± 7.2	9.9 ± 0.3	0.29 ± 0.01
2.25 nM	207	21.8 ± 0.5	125.0 ± 7.3	2.2 ± 0.1	402.9 ± 13.7	5.8 ± 0.2	0.31 ± 0.02
2.5 nM	209	16.2 ± 1.1	127.7 ± 7.6	2.4 ± 0.1	320.6 ± 9.0	8.6 ± 0.4	0.32 ± 0.02
5 nM	228	17.2 ± 1.1	116.2 ± 7.4	1.9 ± 0.1	258.9 ± 6.3	7.8 ± 0.4	0.36 ± 0.02
10 nM	256	16.8 ± 1.0	123.1 ± 7.5	2.5 ± 0.1	353.5 ± 10.1	7.4 ± 0.3	0.37 ± 0.02
25 nM	211	15.6 ± 1.1	102.3 ± 6.9	2.0 ± 0.1	356.3 ± 12.6	6.4 ± 0.3	0.45 ± 0.02
50 nMa	252	24.5 ± 0.8	95.2 ± 6.6	1.5 ± 0.1	513.6 ± 20.7	2.9 ± 0.1	0.47 ± 0.04

Table of growth and catastrophe parameters from all control and KIF3CC titration assays performed herein. The data were compiled from kymograph analysis of the plus-ends of dynamic MT seeds over a period of 10 min. The number of MT seeds used for each condition ranged from 34 to 39 seeds from three replicate days of experiments. All values are reported mean ± SE.

^aData for 50 nM KIF3CC are presented twice on this table.