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. 2017 Aug 28;14(5):4363–4370. doi: 10.3892/etm.2017.5045

Figure 1.

Figure 1.

Effects of AGE and LPS on IL-6 and IL-1β from J774 macrophages. J774 macrophages were incubated for 24 h in the presence of either AGE or LPS. (A) IL-6 levels in supernatant were determined using ELISA. (B) The IL-6 mRNA levels was assessed by RT-qPCR. (C) IL-1β levels in supernatant were determined using ELISA. (D) The IL-1β mRNA levels was assessed by RT-qPCR. Data are expressed as the mean ± standard error of the mean. n=4 for each experiment. *P<0.05 and **P<0.01 vs. BSA, ##P<0.01 vs. control. AGE, advanced glycation end-products; LPS, lipopolysaccharides; IL, interleukin; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; BSA, bovine serum albumin.