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Mononuclear cells isolated from human UCs were cultivated in complete endothelial cell growth medium-2. Cells demonstrated a typical cobblestone appearance. The human UC-derived ECFCs were (A) cultured for 72 h, (B) passaged once and (C) passaged 10 times. Scale bar, 100 µm (magnification, ×100). UC, umbilical cord; ECFCs, endothelial colony-forming cells.
