Figure 3.

Cluster of differentiation CD34⁺ cell expansion with cytokine cocktail and feeder (magnification, ×200). (A) Cells in the (a-f) normoxia or (g-l) hypoxia testosterone and control groups were cultured for (a,d,g,l) 1, (b,e,h,j) 3 and (c,f,i,l) 7 days. Cell proliferation was markedly higher in the normoxia groups compared with the hypoxia groups and addition of testosterone promoted cell expansion in the normoxia and hypoxia groups. (B) Comparison of the total cell growth rate among all groups. The amplification efficiency was significantly increased in the normoxia groups compared with the hypoxia groups and the presence of testosterone increased cell expansion, particularly during the final 4 days under hypoxia. *P<0.05 vs. normoxia control, hypoxia testosterone and hypoxia control groups. ^#P<0.05 vs. hypoxia control group. (C) Comparison of total cell numbers. Cell numbers were significantly increased in the normoxia groups compared with the hypoxia groups. The addition of testosterone increased amplification in normoxia and hypoxia groups. Data are presented as the mean + standard deviation. *P<0.05 and **P<0.01.