Table 2.

Thermodynamic Parameters for the Unfolding Transitions of Horse Heart Ferricytochrome c Variants^a

Variant	pH	Thermal Denaturation		GuHCl-Induced Denaturationb
		Tm (K)	ΔHD (kJ mol−1)	[GuHCl]1/2 (M)	mD (kJ mol−1 M−1)	−ΔGf (kJ mol−1)
WT	7.4	357.1c	360 ± 30c	2.7 ± 0.1d	11.5 ± 2.6d	31.1 ± 7.1d
	4.5	344 ± 1.0	205 ± 30	2.6 ± 0.1	9.8± 0.2	25.1 ± 0.7
T49V	7.4	359 ± 1.6	351 ± 130	2.7 ± 0.1	11.2 ± 3.7	30.2 ± 10.1
	4.5	345 ± 0.8	132 ± 87	2.5 ± 0.2	6.7 ± 1.9	16.7 ± 4.9
Y67R/M80A	7.4	344 ± 1.5	133 ± 4	1.7 ± 0.1	10.3 ± 0.1	17.5 ± 1.0
	4.5	328 ± 0.4	151 ± 1	1.4 ± 0.04	9.6 ± 1.3	13.4 ± 1.9
M80A	7.4	357 ± 0.7	306 ± 79	2.71 ± 0.02	10.3 ± 0.6	27.9 ± 1.6
	4.5	349 ± 1.1	344 ± 55	2.6 ± 0.1	11.6 ± 0.3	30.2 ± 1.4

^aMonitored is ellipticity at 222 nm.

^bPerformed at 22 ± 2 °C.

^cFrom differential scanning calorimetry, pH 7.0, ref. 57.

^dFrom ref. 8.