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letter
. 2017 Sep 6;9(10):2732–2738. doi: 10.1093/gbe/evx173

Fig. 1.

Fig. 1.

—Examples of sequence alignments between Bemisia tabaci “Peru” MEAM1 mtDNA COI haplotype gene region, published “MEAM2” mtDNA COI gene region, and NGS candidate NUMT sequences identified from KX234913, KY951449, and the KY951452 individuals. (A) C-terminal region of a Peruvian MEAM1 B. tabaci (KY951450) mtDNA COI gene region showing putative stop codon (black shaded “*” symbol), as well as the B. tabaci “MEAM2” haplotype from Japan (AJ550177), and examples NGS candidate NUMT sequences from the Peruvian B. tabaci MEAM1 (KX234914) with matching SNPs (indicated by red boxes) that matched the Japan MEAM2 haplotype (AJ550177). Deletion of a “T” base (indicated by red triangle) resulted in a frameshift mutation and the loss of the putative mtDNA COI gene stop codon. (B) Internal stop codons (at positions 904S906) detected in candidate NUMT sequences (KY951452_NUMT-01, KY951452_NUMT-02) from the Peruvian MEAM1 individual (KY951452) MiSeq generated DNA fragments when compared with the Peruvian B. tabaci MEAM1 (KX234914) mtDNA COI gene. Stop codons detected in NUMT sequences were the result of a single nucleotide base change at position 906 from a “T” to an “A.” Candidate NUMT sequences were also compared with the Peruvian MEAM2 haplotype (KY951454) obtained via PCR and Sanger sequencing of the same MEAM1 individual (KY951452). Nucleotide positions based on the mtDNA COI gene are provided. Amino acid translation based on the invertebrate mitochondrial genetic codes (Translational Table_5). Significant changes between amino acids are highlighted.