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. Author manuscript; available in PMC: 2017 Oct 19.
Published in final edited form as: Cell Rep. 2017 Oct 17;21(3):692–706. doi: 10.1016/j.celrep.2017.09.069

Figure 2. Per2 is required for overall S opsin levels but not for maintaining S opsin gradient.

Figure 2

(A) Relative expression of Per2, Per1, Per3 and S opsin transcripts in P24 control and Crx-Cre; Per2Fl/Fl retinas using qRT-PCR. Fold change is relative to expression in the control group. Error bars are ± SEM and n=4. ** indicates P<0.01.

(F–j) P42 retinal flat mount preparations coimmunolabeled with S opsin (green) and M opsin (red) for control (F-left panel) and Per2 conditional mutants (F-right panel). The S opsin gradient is not perturbed in the Crx-Cre; Per2Fl/Fl animals, but decrease in S opsin expression was observed at high magnification, (g–j right panels) compared to the control (g–j left panels). D-dorsal and V-ventral.

(B–E) Quantification of total cones (B), dual S+M opsins cones (C), genuine S opsin cones (D), and genuine M opsin cones (E) in 20x fields across far dorsal (FD), mid dorsal (MD), mid ventral (MV) and far ventral (FV) regions of the retina. Error bars are ± SEM and n=5–6. Results were analyzed using Student’s t-test. * indicates P<0.05.