(A–d) P24 retinal flat mount preparations coimmunolabeled with antibodies to S opsin (green) and M opsin (red) from control (A) and Dio2 mutant (C). Representative high magnification images of the dorsal retina from control (b′) and Dio2 mutant (d′) showing increased density of dual cones in Dio2−/− retina.
(E–H) Quantification of total cones (E), dual S+M opsins cones (F), genuine S opsin cones (G), and genuine M opsin cones (G) in 20x fields across far far dorsal (FFD), far dorsal (FD), mid dorsal (MD), mid ventral (MV), far ventral (FV) and far far ventral (FFV) regions of the retina. Error bars are ± SEM and n=6. Results were analyzed using Student’s t-test. * indicates P<0.05 and ** indicates P<0.01.
(I–L) P42 retinal sections coimmunolabeled with S opsin (green) and M opsin (red) for control (I and J) and Dio2 mutant (K and L) indicating that in the Dio2−/− mutant the phenotype persists in the adults. (l1–4, K1–4) High magnification images of the boxed regions.