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. Author manuscript; available in PMC: 2018 Oct 27.
Published in final edited form as: Vaccine. 2017 Sep 28;35(45):6112–6121. doi: 10.1016/j.vaccine.2017.09.058

Figure 2. Global analysis of the transcriptional responses to WT and antigenically-matched LAIV in differentiated hNEC cultures.

Figure 2

(A and B) Venn Diagrams of overlap in the response to infection with WT and LAIV in hNEC cultures. Number of differentially expressed (DE) genes following virus infection relative to time-matched mocks. Differential gene expression cutoff was set to fold change >1.5 and a q-value < 0.05 calculated using a moderated t-test with subsequent Benjamini-Hochberg correction. Multidimensional scaling (MDS) representation of the similarities in the differential transcriptional profiles elicited by viral infection at (C) 24 hpi (D) and 36 hpi. Each donor is represented by a distinct shape. Biological replicates are represented as single points and unique color distinguishes infectious conditions. The quality of the representation is provided by the Kruskal Stress value, with the low percentage of Kruskal stress suggesting a faithful 2D representation of global transcriptional differences between viral strains. (E) Average ratio of gene expression between LAIV and WT infected cells. Heatmap represents the log2 fold expression changes of 253 genes displaying a 1.2 fold-change of expression in LAIV- over WT-infected cells relative to donor-matched, mock-infected cells.