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. Author manuscript; available in PMC: 2018 Oct 27.
Published in final edited form as: Vaccine. 2017 Sep 28;35(45):6112–6121. doi: 10.1016/j.vaccine.2017.09.058

Figure 4. LAIV infection is associated with enhanced cytokine and chemokine induction in hNEC cultures.

Figure 4

(A) Gene set enrichment of biological functions pertaining to immune cell recruitment and regulation derived from IPA. Bubble plot representation of significant enrichment scores (activation z-score >2) in at least one timepoint in cells infected with LAIV. Significant enrichment is denoted by red color. Activation z-scores < 2 are denoted in white and grey. Crosses signify a lack of enrichment. Bubble diameter represents the −log10 p-value as determined by Fisher’s exact test. (B) Measurement of CXCL10 production in the basolateral medium in infected hNEC cells at 36 hpi. Graphs represent the log2 fold change in cytokine expression relative to donor-matched, mock-infected cells (left). Ratio of CXCL10 expression in LAIV-infected cells relative to WT- infected cells (right). Values greater than zero denote increased cytokine production in LAIV-infected cells relative to WT-infected cells. (C) Differential expression of cytokine and chemokine genes. Heatmap represent the average ratio of differential expression (log2 fold change) relative to donor-matched, mock-infected samples.