Table 1. Correlation of assay results in determining FimH binding and biological activity.

Assay	α-D-mannose	α-methyl mannoside (αMM)	α-heptyl mannoside (HM)
Disaggregation IC50	--	BLQa	∼78 μM
HAI – EC90	>1 mM	>1 mM	15 μM
Aggregometry IC50	--	--	77 μM
Biofilm IC50	--	--	20 μM
DSF Tm (°C)	--	∼64.5	69.8
FP-EC50	--	--	89 μM
Cell-free Binding IC50	--	--	73 nM
Radiolabel KD	4.1×103 nM	2.4×103 nM	32 nM
SPR KD	2.3×103 nM	2.2×103 nM	5 nM
BLI KD	--	61 nM	--
ELLSA IC50	--	--	0.16 μM

^aBLQ, below level of quantification