Gene locus
diagrams for nonannotated E. coli proteins
YmcF (A), YnfQ (B), YnaL (C), and YhiY (D). Line represents
chromosomal DNA, annotated protein-coding sequences are represented
by gray boxes, and newly reported coding sequences are represented
by blue boxes. Arrows indicate 5′–3′ directionality
of the coding sequence. Sizes are proportional to length, and genomic
coordinates of novel protein sequences are provided. Sizes of novel
proteins were calculated either from the first in-frame ATG to stop
codon (C, D) or from experimentally determined non-ATG start codons
(A, B, vide infra).