Table 1.
Gene | Primer | Sequence (5´–3´) | |
---|---|---|---|
GLU | Primer 1 | CTAGCTAGCATGGGCTATCAAGCCAAAG | Nhe I |
GLU | Primer 2 | CGCGGATCCGCTTCCTCCTCCTCCGCTTCCTCCTCCTCCGCTTCCT | BamHI |
CCTCCTCCAATCCGAACTCGTTCTCCAG | |||
FTH | Primer 3 | CGCGGATCCATGACGACCGCGTCCACC | BamHI |
FTH | Primer 4 | CCGCTCGAGTTAGCTTTCATTATCACTGTC | XhoI |
Restriction enzyme recognition sites are italicized.