Figure 1.

Effects of Aux and CK on the expression of cell cycle genes in grape somatic embryos. Grape somatic embryos (GSE) mostly at the globular or heart-shape stage were transferred from subculture of semi-solid growth regulator free X6 medium to liquid X6 medium and incubated with 10 µM indoleacetic acid (IAA), 2 µM benzilaminopurine (6-BPA) and water as control, respectively. Samples were shaken at 115 rpm for 72 h. Afterwards, the liquid medium was discarded and the GSE were frozen until used. Gene expression analysis was performed by RT-qPCR normalized to VvUBIQUITIN. Values are referred to control samples and correspond to the average of 3 biologic replicates, bars represent s.d. and asterisks indicate significant differences with respect to control samples (Dunnett´s multiple comparison test p < 0.05).