Figure 2.

N-terminal signal peptide is required for rescue of cyp85a2-2 mutant phenotype. A. Rosette leaf morphology of Col-0, cyp85a2-2, Cyp::RFPCYP85A2-CSPY/cyp85a2-2 and Cyp::RFPCYP85A2-SSPY/cyp85a2-2. B. Western blot showing RFP-CYP85A2 accumulation in Col-0 controls, Cyp::RFPCYP85A2-CSPY/cyp85a2-2 and Cyp::RFPCYP85A2-SSPY/cyp85a2-2 in different membrane fractions. * Non-specific signal was detected with anti-RFP. C. Bundle sheath cells expressing RFP–CYP85A2 in either CSPY (top) or SSPY (bottom) seedlings. Chloroplast autofluorescence can be seen in blue; merge indicates merged channel between the RFP fluorescence and chloroplast autofluorescence. Scale bars = 10 μm