Fig. 3.

Virus titer reduction assay. NCI-H358 cells were infected with MOI 0.2 of wild type NiV (A) or HeV (B) for 1 h at 37 °C. After 1 h incubation, virus inocula were removed, cells were washed in PBS, and media containing 2-fold serial dilutions of R1479 was added to cells. Cells were allowed to incubate for 48 h, at which time supernatant was removed and used to determine TCID₅₀ on Vero cells. Each experiment was performed in quadruplicate, and the calculated EC₅₀ values are displayed. The dotted line at 37.7 TCID₅₀/mL is the lower limit of detection, and the dotted line at 3.16 × 10⁷ TCID₅₀/mL is the upper limit of detection.