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. Author manuscript; available in PMC: 2018 Jul 28.
Published in final edited form as: ACS Sens. 2017 Jun 16;2(7):961–966. doi: 10.1021/acssensors.7b00223

Figure 2.

Figure 2

Genetic incorporation of p-vinyloxy-L-phenylalanine (ViP). (A) The structure of ViP; (B) SDS-PAGE analysis of sfGFP-Asn149TAG mutant (arrow) expressed either in the absence (lane 1) or in the presence (lane 2) of 1 mM ViP; (C) Fluorescence readings of cells expressing PrFRS and sfGFP-Asn149TAG mutant. The expressions were conducted either in the presence or in the absence of 1 mM ViP. Fluorescence intensity was normalized to cell growth; (D) Deconvoluted ESI-MS spectra of the sfGFP-Tyr66ViP mutant. Expected masses: 27315.5 Da (without the N-terminal Met) and 27446.7 (with the N-terminal Met); observed masses: 27315.4 Da (without the N-terminal Met) and 27446.6 Da (with the N-terminal Met). The other signals do not correspond to sfGFP mutant that contains tyrosine (the major background incorporation; 27290.7 or 27421.7) or any other proteinogenic amino acids at position Tyr66.