FIGURE 1.

(A) Cumulative growth of BF and PF CN cells in which the tet-regulatable WT KREPB4 allele was expressed (E) or repressed (R). (B) RT-qPCR analysis of BF and PF CN cells in which the tet-regulatable WT KREPB4 allele was expressed (E) or repressed (R) for 2 and 4 d, respectively. The abundances of KREPB4, and never-edited (COI and ND4), pre-edited, and edited mitochondrial mRNAs in repressed cells were calculated relative to cells expressing WT KREPB4, and plotted on a log scale. For each target amplicon, relative abundance was determined using TERT as an internal control. Data represent mean of three experiments, and error bars indicate mean ± SEM. Asterisks indicate that the target amplicon was not detected in the repressed cells.