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. 2017 Nov;23(11):1672–1684. doi: 10.1261/rna.062786.117

FIGURE 2.

FIGURE 2.

(A) Western analysis of total lysates (equivalent of 1 × 107 BF cells/lane, and 5 × 106 PF cells/lane) from BF and PF CN cells in which the tet-regulatable WT KREPB4 allele was expressed (E) or repressed (R) for 2 and 4 d (BF), and 4 and 8 d (PF). The Western blots were probed with monoclonal antibodies against editosome proteins KREPA1, KREPA2, KREL1, and KREPA3, the MRB1 complex component GAP2, and mitochondrial Hsp70. (B) Western analysis of 5 h 10%–30% glycerol-gradient fractions of lysates from BF and PF CN cells in which KREPB4 was expressed or repressed as in A.