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. 2017 Nov;23(11):1672–1684. doi: 10.1261/rna.062786.117

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© 2017 McDermott and Stuart; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 6. — Western analysis of 5 h 10%–30% glycerol-gradient fractions of lysates from (A) BF and (B) PF CN cells constitutively expressing V5-tagged WT, G163R or G163V KREPB4 alleles, and in which the tet-regulatable WT KREPB4 allele was expressed (E) or repressed (R) for 2 (BF) or 4 (PF) days. Western blots were probed with monoclonal antibodies against editosome proteins KREPA1, KREPA2, KREL1, and KREPA3, and with anti-V5-tag antibody to detect the constitutively expressed V5-tagged WT or mutant KREPB4 proteins.