FIGURE 3.

The HAdV-37 MLP-TSS-sRNAs are capped and enriched in Ago2-containing RISC. (A) Northern blot analysis of total cytoplasmic (Cyto) and Flag–Ago2-associated (F-Ago2-IP) RNA. The membrane was probed sequentially for MLP-TSS-sRNA and 5′ mivaRNAII. The lower panel is a Western blot for Flag–Ago2 and Actin in the cytoplasmic and Ago2-bound fractions. (B) Northern blot analysis demonstrating that the Ago2-associated MLP-TSS-sRNAs contain a 5′ cap structure. Ago2-associated RNAs were used for immunoprecipitation with an m7G-cap antibody. The membrane was sequentially probed for MLP-TSS-sRNA, 5′ mivaRNAII and let7b.