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. Author manuscript; available in PMC: 2017 Oct 19.
Published in final edited form as: Bioconjug Chem. 2017 Feb 15;28(4):933–943. doi: 10.1021/acs.bioconjchem.6b00660

Figure 3.

Figure 3

Comparison of the Exo III-based and the DTT displacement assays with SH probe DNA-conjugated AuNPs prepared at different DNA:AuNP ratios. (A) Fluorescence intensities of supernatants after Exo III digestion (black) and DTT displacement (red). (B) Standard calibration curve obtained with known concentrations of FAM-labeled, thiolated probe DNA after Exo III digestion (black) and DTT displacement (red). (C) Surface coverage of SH probe DNA-modified AuNPs as characterized by Exo III digestion (black) and DTT displacement (red). Error bars show standard deviations obtained from three measurements.